Many studies have reported increases in the risk of acute cardiovascular events following daylight savings time (DST) transitions. We sought to investigate the effect of DST transition on the incidence of out-of-hospital cardiac arrest (OHCA).Between January 2000 and December 2020, we performed an interrupted time series analysis of the daily number of OHCA cases of medical aetiology from the Victorian Ambulance Cardiac Arrest Registry. The effect of DST transition on OHCA incidence was estimated using negative binomial models, adjusted for temporal trends, population growth, and public holidays.A total of 89,409 adult OHCA of medical aetiology were included. Following the spring DST transition (i.e. shorter day), there was an immediate 13% (IRR 1.13, 95% CI: 1.02, 1.25; p = 0.02) increased risk of OHCA on the day of transition (Sunday) and the cumulative risk of OHCA remained higher over the first 2 days (IRR 1.17, 95% CI: 1.02, 1.34; p = 0.03) compared to non-transitional days. Following the autumn DST transition (i.e. longer day), there was a significant lagged effect on the Tuesday with a 12% (IRR 0.88, 95% CI: 0.77, 0.99; p = 0.04) reduced risk of OHCA. The cumulative effect following the autumn DST transition was also significant, with a 30% (IRR 0.70, 95% CI: 0.51, 0.96; p = 0.03) reduction in the incidence of OHCA by the end of the transitional week.We observed both harmful and protective effects from DST transitions on the risk of OHCA. Strategies to reduce this risk in vulnerable populations should be considered.